Eppendorf Innova 43R Incubator Shakers - Manual (Page 45)

45
New Brunswick Operating manual
Whatever step is shown on the right-hand side of the display is settable (in Figure
23, it is Step 2). To scroll between steps, select the arrows (bottom left), then turn
the Control Knob clockwise or counterclockwise, then select the desired step.
NOTE:
The time entered for each step is for that step only; it is not cumulative (i.e.,
the elapsed time from the start of the program).
12. Continue to program as many as 15 steps in the same manner. For our sample
program, we have only three steps (see Figure 24).
Figure 24: Program 1 – Step 3
PRG1 - STEP 02 03
Time 03:00 12:00
ºC 40.0 4.0
RPM 225 0
UV Off Off
GRO Off Off
 Save Cancel
The three-step program we have illustrated is designed to start incubating cultures
at 37ºC and hold that temperature for eight hours, shaking at 150 RPM. After
eight hours, Step 2 engages, increasing the temperature setpoint to 40ºC for a
temperature induction, holding that temperature for three hours and increasing the
shaking speed to 225 RPM. After this interval, the temperature will be cooled to
4ºC and held at that temperature for twelve hours; no shaking will occur during
this period, as the speed is set to 0 RPM.
Please note that temperature reductions, even at temperatures above ambient
(going from 40º to 30ºC, for example), require refrigeration to be effective.
To save the entire program:
1. Once all your steps are set, use the Control Knob to highlight
Save at the
bottom of the screen, then click the Knob in. The field will flash.
2. Click the Knob in again to save the program. The display will read Process
Running – Saving Profile for a few seconds, then return to the main Programs
( PROG ) screen.
If you wish, you can set Programs 2, 3 and 4 and save them in the same manner.