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4 ph calibration with a user-defined buffer, Ph calibration with a user-defined buffer – Thermo Fisher Scientific CyberScan DO 600 User Manual

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2.4 pH Calibration with a User-defined Buffer

If you selected ‘USER’ (Custom) buffer in the pH Setup, the following screen is
shown when you enter calibration mode. You need to prepare at least 2 custom
buffers of known pH values.

Figure 18 : User-define Buffer Screen

1. Rinse the electrode in clean water and prepare electrode for calibration.

2. Dip the probe in custom calibration buffer. The tip of the probe must be

completely immersed into the solution. Stir the probe gently to create a
homogeneous solution.

3. The screen shows 2 readings. The upper display shows the pH reading of

the solution with respect to previous calibration (if any) & lower display
shows the pH reading of the solution without any calibration. Use &

keys to adjust the upper display to the pH value of your custom buffer.

4. Press ENTER key to confirm the calibration.

5. Rinse the probe with clean water. Place it in the next buffer and follow steps

2, 3 and 4 to calibrate other points.

Function Keys available in pH Calibration mode:

TEMP (F1)

Goes to temperature calibration

CLR-C (F2)

Clears previous calibration (if any) after ENTER key is pressed for the
confirmation screen

NEXT (F3)

Shows calibration report

Note: Custom buffer solution values should be at least 1 pH unit apart
from each other. Otherwise, the meter will not accept the buffer values.

Note: If you wish to completely re-calibrate the meter, you need to clear
previous calibration data. Press CLR-C (F2) key to clear previous
calibration. The meter shows you confirmation screen. Press ENTER key
to confirm deleting previous calibration.