1 ph calibration points, 3 ph calibration with a standard buffer, 1 to start ph calibration – Thermo Fisher Scientific CyberScan DO 600 User Manual
Page 53: Ph calibration points, Ph calibration with a standard buffer, To start ph calibration

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Buffer
Group
First Point of Calibration
(when complete re-calibration)
Other Available Calibration Points
USA
pH 7.00
pH 1.68, 4.01, 10.01, and 12.45
NIST
pH 6.86
pH 4.01, 6.86, 9.18, and 12.45
DIN
pH 6.79
pH 1.09, 3.06, 4.65, 9.23, and 12.75
PWB
pH 6.97
pH 4.10
Table 1: Buffer Groups and Calibration Points
The meter automatically recognizes and calibrates to these standard pH buffer
values, which makes pH calibration faster and easier.
2.2 pH buffer group for calibration and auto-recognition
This option allows you to choose a buffer group from four internationally
recognized standard buffer groups namely USA, NIST, DIN & PWB. The default
buffer group is USA. Refer to the ‘pH Setup’ section (pH Parameter guide) for
information on how to set the meter to a particular buffer group.
2.2.1 pH Calibration points
This option allows you to calibrate up to 6 points, depending on the standard
buffer selected. Or you can choose to have custom buffers with 2 to 5 points.
When completely re-calibrating the meter, the first point of calibration should be
one of the following, depending on your choice of standard buffer group. The
meter automatically recognizes and calibrates to the standard pH buffer values,
which makes pH calibration faster and easier.
2.3 pH Calibration with a Standard Buffer
Make sure you have selected a standard buffer with which you intend to
perform pH calibration.
2.3.1 To start pH Calibration:
1. Switch the meter on and make sure the meter is in pH measurement mode.
2. Press CAL (F2) to start calibration.
3. The meter shows the ‘Calibration-Rinse Electrode’ screen for a few seconds
and then shows the pH calibration screen.
Note: If the meter is password protected, you will be prompted to enter a
password.