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Luminex 100 IS User Manual, Version 2.1 User Manual

Page 39

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MAP Technology

Installation

PN 89-00002-00-070 Rev. A

4 - 11

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To adjust the vertical height of the Sample Probe:

1. Turn on the XYP instrument, the Luminex 100 analyzer, and PC.

Wait for the Luminex 100 IS software to start.

2. Remove the clear plastic shield that covers the sampling probe

area.

3. In a 96-well microtiter plate where overall height is no more than

0.75 inches, place appropriate alignment tool in the plate.

For a standard plate with flat-bottomed wells, stack two of the
larger (5.08 mm diameter) alignment discs together and place
into well A1.

For a half-volume plate with flat-bottomed wells, stack two of
the smaller (3.35 mm diameter) alignment discs together and
place into well A1.

For a plate with conical wells, place one alignment sphere into
well A1.

4. Click Eject/Retract to eject the plate. Place the 96-well

microtiter plate on the plate with position A1 in the top left
corner and click Eject/Retract to retract the plate.

5. Use the 3/32 inch hexagonal allen wrench to loosen the height

adjustment locking screw. See Figure 24.

6. Click the Maintenance tab.

7. Click the Sample Probe Down button to lower the Sample

Probe.

8. Using the thumb wheel, lower the probe until it is just touching

the top of the alignment discs or sphere.

9. Use a 3/32 inch hexagonal allen wrench to tighten the height

adjustment locking screw.

10. Click Sample Probe Up to raise the sampling probe.

11. Replace the clear plastic shield that covers the sampling probe

area.