Luminex 100 IS User Manual, Version 2.1 - Manual (Page 117)

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PN 89-00002-00-070 Rev. A 7 - 53
2. Select the template that uses the product for which you are
assigning a dilution factor and click Open. A Luminex Batch
Setup dialog box appears.
3. Scroll down the template command list to the item that uses the
dilution factor that you are changing.
4. Click the Dilution Factor field, which is white to indicate that
you can enter text into the field.
5. Enter the dilution factor used if the factor is other than 1. Enter
each dilution factor as a decimal, not as a ratio.
6. Click Finish. The system saves the changes you made.
Start a Plate The start plate command begins the acquisition process. During
acquisition, the instrument takes up sample from the microtiter plate
and processes it.
‹ To start acquiring data from sample:
1. Check the Sample Probe vertical height adjustment and adjust as
needed.
2. Create or open the batch that you want to process.
3. Click Eject/Retract to place the microtiter plate on the plate
holder.
4. Prepare the microtiter plate with sample for processing.
5. Load the microtiter plate on the plate holder, with well A1 in the
upper-left corner. Be attentive to follow the kit manufacturer’s
directions.
6. Click Eject/Retract again to retract the plate holder.
7. Click Start Plate from the Run Batch tab. The system begins
processing the batch’s template commands and acquiring sample
from the wells as the commands appear on the template.
The system takes a specified volume for testing. A horizontal
progression bar appears on the main screen to show the status of
each sample acquisition. After each sample is analyzed,
approximately 160 µL of sheath fluid is dispelled into each well.
As the system analyzes each well, the well shows either a check
mark in a green well for successful acquisitions or an X mark in a red