Bio-Rad Bio-Plex Manager™ MP Software Upgrade - Manual (Page 36)

3 | Running Protocols
34 | Bio-Plex Manager MP Software
The x-axis is the Classification 1 channel and the y-axis is the Classification 2
channel. These channels measure the embedded fluorochromes in each bead,
which are used to identify the bead set and corresponding analyte. The resulting
data point clusters in the map represent the different bead sets in the assay and
their associated analytes.
Tip: Use Maximize View to expand the bead map to fill the entire window.
Once maximized, you can use the Zoom tool to focus further on a particular
area in the bead map.
Note: Each bead set generates a cluster of data points, rather than a single
point, because of minute variations in fluorochrome levels and intensity among
the beads in the set.
The white areas on the map indicate the expected regions of the selected analytes.
When you move your cursor over these areas, the name of the analyte appears in a
tool tip. The data point clusters should fall within these regions. If one or more
clusters do not fall within the white areas, there may be a problem with the beads
such as photobleaching. Or the analyte selections in the protocol may be incorrect.
If all the data clusters have shifted out of the white regions, there may be
microbubbles in the cuvette. The bead map can be used to confirm that the bead
sets are correctly selected, measured, and identified.
Interrupting a Run
The results for each run, whether the run is completed or interrupted, are saved to a
unique result which can later be retrieved and viewed. Any data that were read up to
the point the run is interrupted are saved to the result name you specify in the Run
Settings dialog box. When you are ready to run the protocol again, return to the Run
Settings dialog box.
To interrupt a run
 Click Stop to interrupt a run.