Bio-Rad Bio-Plex Manager™ MP Software Upgrade - Manual (Page 18)

2 | Creating Protocols
16 | Bio-Plex Manager MP Software
Well Types
The following are different well types that you can specify on the plate:
Blank Standard Undefined
Control Unknown
 Blank Wells
In certain types of assays, such as the Bio-Plex phosphoprotein assay, it might
be useful to subtract the assay background from the readings of standards,
controls, and unknown samples. Prepare blank wells that contain all of the
assay components excluding the sample. Blank wells are read along with the
rest of the assay. Bio-Plex Manager 6.x then subtracts the mean background
reading of these wells from the fluorescence intensity values of the wells
containing standards, controls, and unknowns.
 Control Wells
Control wells contain samples of known concentration. The observed
concentration of the control wells is compared to the expected concentration
and calculated at the end of the reading.
 Standard Wells
Standard wells contain analytes of known concentration. A series of known
concentrations of an analyte is used to generate a standard curve of
fluorescence intensity compared to analyte concentration. The regression
equation for the curve is used to calculate the concentration of analytes in the
unknown samples and controls.