beautypg.com

Bead map – Bio-Rad Bio-Plex Manager™ MP Software Upgrade User Manual

Page 35

background image

Bead Map

User Guide

| 33

your analytes. The new analytes, if detected during the run, will then appear in

the table.

Column Heading

Description

Well

Location of the well on the plate template.

Type

Identifies the type of well, Blank, Control, Standard,
Unknown, and the sample. For example, C1 is the first
control sample and S4 is the fourth standard sample.

Total Events

Total bead events recorded for that well, including
unclassified events (events that fall outside the 50
regions). This column can be displayed or hidden
using a toggle command.

Total Region
Events

Total bead events recorded within the 50 defined
regions. This column can be displayed or hidden
using a toggle command.

Region_##(##)

When bead region counts are displayed, there are two
numbers. The first number is the fluorescence
intensity for that analyte. The second number, in
parentheses, is the number of bead events for that
region.

If there are low bead counts for any of the selected
analytes, the following symbols appear:

*

There are fewer than the recommended
50 beads for one or more analytes.


There are fewer than 20 beads for one or
more analytes.

Bead Map

The bead color map is a density dot plot of the events in a reading for a selected

well. Different colored dots in the map represent different numbers of events at

those data points. There are 50 available regions in the bead map.

This manual is related to the following products: