Bio-Rad Bio-Plex Manager™ MP Software Upgrade - Manual (Page 14)

2 | Creating Protocols
12 | Bio-Plex Manager MP Software
About Analytes
Bio-Plex Manager MP software groups analytes by panels. Preconfigured panels of
analytes that correspond to off-the-shelf Bio-Plex® assays can be selected. These
assays include human, mouse, and rat cytokines and phosphoproteins, as well as
the newer human angiogenesis, diabetes, isotyping, and acute phase assays. The
following assays are currently not available for the MAGPIX instrument: nonhuman
primate diabetes, canine kidney tox, rat and human kidney tox, angiogenesis, and
acute phase. You can add other panels to this list or create custom panels that
include only the analytes you use in your experiments.
Note: After you select the assay panel, you select which analytes you want to
appear in your reports. During a reading, the array reader detects all the
analytes in the sample, including any analytes you excluded from selection.
However, only the selected analytes are included in the final reports and tables.
After the run is completed, you can always edit the protocol, select any
analytes that were previously omitted, and the data for those analytes will
appear in the tables.
Each analyte is listed by name and region. The region number refers to the area of a
fluorescent color map that identifies the analyte’s bead region. Each bead region is
embedded with specific quantities of two fluorescent dyes. The array reader detects
the combination of these fluorochromes and associates the bead region with a
unique region on the color map. Therefore, the location on the map identifies the
bead region and its associated analyte.
Each analyte on a Bio-Rad preconfigured panel is identified by a unique region.
However, analytes on different panels may occupy the same region. If you pick
analytes from more than one panel, only one analyte for a particular region can be
selected. Bio-Plex Manager MP warns you if there is a conflict.