Eppendorf InjectMan NI 2 - Manual (Page 31)

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5.2.5 Field of movement
To utilise the benefits of axial injection to the full, only one motor (A module) is used. This
permits less harmful injection, with minimal damage to the cell.
As a result, vertical movements are set with the A module and the X axis together. To perform
a downward movement, the X axis is moved outwards and the A module is moved forwards,
simultaneously. This results in the downward Z movement.
This has effects on user control of the micromanipulator in the boundary zones of the
X axis/Z axis.
In the case of a three-dimensional manipulator with a motor standing for each coordinate, the
field of movement forms a cube with equal sides.
As a result of the arrangement of the motor axes of the InjectMan ® NI 2, this cube is tilted in
X direction by the mounting angle of the A module. Consequently, it is not possible to execute
purely Z movements at the sharp angled corners of the cube. Here axial movements are
necessary, executed by simultaneously moving the joystick back and forth and rotating it.
The actual field of movement (provided the capillary is mounted properly) relevant to the
experiment is much smaller than the tilted cube. Within this working area, conventional working
in the three coordinates is possible.
Fig. 8: a) Cartesian field of movement b) Field of movement with axial component
1 Capillary
2 Field of movement in experiment
3 Height of capillary
4 Position of capillary after start of
CentrMot function (section 5.4.2.7)
Z
Y
X
1
3
2
4
User control and mode of operation
5 User control and mode of operation
5
05_Bedien_en.fm Seite 84 Donnerstag, 6. Dezember 2012 1:34 13