4 multipoint calibration – Metrohm 714 IC Metrodata User Manual

Page 35

3.4 Multipoint calibration

IC Metrodata for Win95

3.4 Multipoint

calibration

This section describes the procedure how to perform a calibration

with multiple standards using either an existing or an user-developed

method and to save this multipoint calibration as a new method.

Preparations

•

Insert separating column in IC system and condition it.

•

Select the full scale range at the 732 IC Detector so that all peaks

of the standard with the highest concentration will be inside the

measuring range.

•

Prepare standards and sample.

Record first standard chromatogram

•

If you wish to develop a new method based on a standard method

proceed according to section 3.1 (without sample recording).

•

If you wish to use an existing example method proceed according

to section 3.2 (without sample recording).

•

In both cases, you receive the new method

*.mtw

and the first

standard chromatogram

*.chw

Record second standard chromatogram

•

Click on

or select

Load method and run

from the

Acquire

menu.

•

Select the method saved on recording the first standard chroma-

togram in the

File Open

window and click the

button.

•

In the

Start Run

window, enter a name as

Ident

to characterize the

standard (max. 14 characters, e.g. "standard #").

•

Click the

button.

The window for the on-line display of the chromatogram opens.

The status display shows the message

Waiting

•

Set injection valve at 733 IC Separation Center to "FILL" (manually

or by program) and fill sample loop with standard.

•

Set injection valve at 733 IC Separation Center to "INJECT" (manu-

ally or by program). The data acquisition is started automatically

and stopped as soon as the analysis time has passed. The chro-

matogram is saved and deleted in the chromatogram window. The

status display shows the message

Waiting

again.