Waters IC-Pak Column and Guard - Manual (Page 8)

[ Care and Use ManUal ]
IC-Pak Column and Guard Column 8
5. Fill the flask to the mark with 18 megohm water. Tables 8 and 9
contain the species concentrations in the monovalent and divalent
cation working standards.
Table 8: Monovalent Cation Working Standard Concentrations
Table 9: Divalent Cation Working Standard Concentrations
e. Running the Calibration Standard and Determining
Column Efficiency
Refer to Table 10 for the parameters to run the calibration standard.
Table 10: Calibration Standard Parameters
* By half-height method on a low dispersion Waters ion
chromatograph
** Do not exceed a flow rate of 1.0 mL/min for the IC-Pak A HR
column. Flow rates higher than this may damage the column and void
the warranty.
f. Calculating Efficiency
Refer to Figure 6 for the appropriate peak to measure for your anion
or cation column. Use the equation in the figure below to compute
column efficiency for comparison to the value in the table.
Figure 6: Half Height Method Test Calculations
Monovalent Cation
Stock Solution ppm
Lithium 0.1
Sodium 0.5
Ammonium 1.0
Potassium 1.0
Divalent Cation
Stock Solution ppm
Magnesium 2
Calcium 4
Strontium 6
Barium 16
Column
Name
Optimal Flow
Rate (mL/min)
Test Sample Efficiency
(Plates)*
Peak for Plate
Measurement
IC-Pak A 1.2 Anion 800 sulfate
IC-Pak C 1.2 Cation 1100 sodium
IC-Pak A HC 2.0 Anion 1500 sulfate
IC-Pak A HR 1.0** Anion 2500 sulfate
IN
JE
C
T
W½
½h
h
= Column Efficiency (plates)
= Volume to peak apex (µL)
= Volume of peak at ½ peak height (µL) N
VR
W ½
N = 5.54 x
2