Waters ACQUITY UPLC M-Class Columns - Manual

[ CARE AND USE MANUAL ]
INT RODUCTION
Waters ® ACQUITY UPLC ® M-Class Columns are manufactured
to exacting specifications and are designed for use at pressures
up to 15000 psi. In order to achieve maximum performance, it is
important to ensure that proper connections are made to minimize
peak tailing and poor efficiency.
Each ACQUITY UPLC M-Class Column is individually tested to
ensure that it passes stringent quality control. Compared to
standard flow chromatography, successful day-to-day performance
of capillary-flow systems can require extra attention to detail.
This document provides several essential recommendations for
the successful use of ACQUITY UPLC M-Class Columns.
ACQUITY UPLC M-Class Columns
ACQUITY UPLC M-Class System. ACQUITY UPLC M-Class Columns are available in 75, 100, 150, and 300 μm I.D.s.
TABLE OF CONT ENTS
I. P REPARING ELUENTS
II. SAMPLE P REPARATION
III. PREPARING AND CONNECTING 75, 100, AND
150 µM I.D. ACQUITY UPLC M-CLASS COLUMNS
IV. P REPARING AND CONNECTING 300 µM I.D.
ACQUIT Y UPLC M-CLASS COLUMNS
V. CARE WHEN STOPPING FLOW
VI. HOW TO DIAGNOSE AND ADDRESS ABNORMALLY
HIGH BACKPRESSURE AND LEAKS
VII. CHECKING AND CLEANING A FOULED EMITTER
VIII. LONG TERM COLUMN STORAGE
IX. ORDERING INFORMATION
Note: Gloves should be worn for ALL operations
detailed in this document.