Waters Protein-Pak SEC Columns - Manual

[ CARE AND USE MANUAL ]
Protein-Pak SEC Columns 1
I. INTRODUCTION
This Care and Use Manual contains information pertaining to the use of
the following Protein-Pak™ SEC Columns:
Protein-Pak 60 7.8 mm (i.d.) x 30 cm, Steel (P/N
WAT085250)
Recommended for use with biopolymers such as peptides, polypeptides,
steroids and small proteins. The pore size distribution provides
excellent separation in the following molecular weight ranges: Native
Globular 1000-20,000 and Random Coil 600 - 8000. Shipped in
100% methanol.
Protein-Pak 125 7.8 mm (i.d.) x 30 cm, Steel (P/N
WAT084601)
Especially suited for separation of biopolymers such as proteins and
enzymes in the following molecular weight ranges: Native Globular
2000 - 80,000 and Random Coil 1000 - 30,000. Shipped in
100% methanol.
Protein-Pak 300 SW 7.8 mm (i.d.) x 30 cm, Steel (P/N
WAT080013)
Protein-Pak 200 SW 8.0 mm (i.d.) x 30 cm, Glass (P/N
WAT011786)
Protein-Pak 300 SW 8.0 mm (i.d.) x 30 cm, Glass (P/N
WAT011787)
The columns of choice for use with larger biological active molecules
in the molecular weight range of Native Globular 10,000 - 400,000
and Random Coil 2000 - 150,000. Shipped in 0.05% sodium azide in
water solution.
CONTENTS
I. INTRODUCTION
II. PREPARATION FOR OPERATION
a. Steel Column Installation
b. Glass Column Installation
c. Solvent Requirements
d. Buffers
e. Protein Denaturants
f. Equilibration
g. Column Purge Volumes
III. CARE AND USE
a. Sample Preparation and Filtration
b. Precautions
c. Storage Consideratons
IV. TROUBLESHOOTING
a. Service and Applications Information
b. Column Efficency
c. Test Conditions
PROTEIN-PAK SEC COLUMNS