Waters XBridge BEH Glycan, 2.5 μm XP and 3.5 μm Columns and Standards - Manual

CONT ENTS
I. INT RODUCTION
II. GET TING START ED
a. Column Installation
b. Column Equilibration
c. Initial Column Efficiency Determination
d. Useful Functional Tests for Benchmarking
a New Column
e. Scalable UPLC and HPLC BEH Glycan
Column Offerings
III. COLUMN USE
a. Sample Preparation
b. Operating pH Limit
c. Solvents
d. Pressure
e. Temperature
IV. T ROUBLESHOOTING
V. COLUMN CLEANING, REGENERATING,
AND STORAGE
a. Cleaning and Regeneration
b. Storage
XBridge BEH Glycan, 2.5 µm XP and 3.5 µm Columns and Standards
I. INT RODUCTION
Thank you for choosing a Waters XBridge ® BEH Glycan Guard or
Column containing our 2.5 µm XP or 3.5 µm particles designed for
HILIC separations of 2-aminobenzamide (2-AB) or 2-aminobenzoic
acid (2-AA) labeled released glycans. This column chemistry, when
used on an appropriately configured LC instrument, is capable
of separating both neutral and charged labeled glycan species.
Retention of 2-AB or 2-AA labeled oligosaccharides is based on
the hydrophobicity of the molecule, a parameter that is broadly
related to hydrodynamic volume or molecular size. The resolving
power of these columns is due in part to the particle size of the fully
porous packing materials. Chemical and mechanical stability of
the column are the consequence of Waters ethylene bridged hybrid
(BEH Technolgy™) particle composition.
The column may be calibrated using Waters Dextran Calibration
Ladder Standard (PN 186006841), such that elution may be
expressed in terms of glucose units (GU). Under the suggested
chromatographic conditions, the retention of 2-AB labeled
oligosaccharides on the XBridge BEH Glycan 2.5 µm XP or 3.5 µm
column may be predicted based on the hydrophilic contributions
of the individual constituent monosaccharides.
[ CARE AND USE MANUAL ]