Waters IC-Pak Column and Guard - Manual (Page 2)

[ Care and Use ManUal ]
IC-Pak Column and Guard Column 2
Table 2: Cation Column Characteristics
The packing material is a styrene divinylbenzene resin with a
sulfonic acid functional group. The columns are shipped in 2 mM
nitric acid.
II. InstallatIon
Before attaching the column in the flow path:
1. Directly connect the HPLC injector to the detector by replacing the old
column with a zero-dead-volume union.
2. Flush the lines free of microparticulates and previous solvents. Flush
the injector loop if applicable.
3. Remove the union.
4. Install the column.
a. Installing a Stainless Steel Column
Remove the end plugs from the column and save them for use when
the column is removed from the system and stored. The column
outlet is indicated by an arrow on the label (showing the direction
solvent should flow).
To install the column, thread the inlet and outlet fittings into the
column until finger tight, and then tighten the fittings 1/4-to-1/2
turn. Do not over-tighten. Over-tightening will damage the
connection. A properly prepared and assembled compression fitting
in good condition is all that is required.
Prepare a new tubing/ferrule connection (Figure 1) when a new
column is connected or when a damaged compression screw or worn
ferrule are removed.
Note: The tubing distance beyond the ferrule may differ for different
column types. Re-size the tubing to the correct distance by replacing
the ferrule.
To prepare a new tubing/ferrule connection:
1. Scribe the circumference of the tubing at the desired break using either
a file with a cutting edge or a tube cutter.
2. Grasp the tubing on both sides of the scribe mark with cloth-covered
pliers (to prevent marring the tube surface), and gently work the tube
back and forth until it separates.
Note: Ensure that the tubing end is straight, open, and free of burrs.
3. Slide the compression fitting, followed by the ferrule (large end of the
taper first) over the tube.
Note: Properly bottom the tubing in the fitting seat. Otherwise, dead
volume could result in sample band spreading.
Figure 1: Ferrule and Compression Assembly (Stainless Steel Column)
b. Installing Non-Metallic Columns
Remove the end plugs from your column and save them for use when
the column is removed from the system and stored. The column
outlet is indicated by an arrow on the label (showing the direction
solvent should flow).
To install the column, thread the inlet and outlet fittings into the
column until finger tight, and then tighten the fittings 1/4-to-1/2
turn. Use caution with non-metallic columns. Over-tightening these
fittings may cause damage which results in a leaking connection.
A properly prepared and assembled compression fitting in good
condition is all that is required.
Cation Column Dimensions Particle Size Capacity
IC-Pak C 4.6 x 50 mm 10 µm 12.0 ± 0.2 µeg/mL
Guard 4.6 x 50 mm n/a 2.0 ± 0.2 meg/mL
COMPRESSION SCREW OR NUT
TUBE
FERRULE
END MUST BE STRAIGHT
AND SMOOTH TO ACHIEVE
MAXIMUM COLUMN EFFICIENCY
CRITICAL DISTANCE TO BE DETERMINED BY
THE UNION OR COLUMN FITTING, ETC.