Waters IC-Pak Column and Guard - Manual (Page 3)

[ Care and Use ManUal ]
IC-Pak Column and Guard Column 3
To replace a fitting:
1. Use a sharp razor blade to make a long tapering cut in the tubing as
shown in Figure 2. Alternately, if tubing is not in short supply, stretch
the tubing (after warming over low heat) between two sets of pliers and
cut the tube at thinnest point. The resulting taper will slip through the
fittings more easily.
2. Pass the tubing through the compression screw and ferrule. Be sure the
stainless steel side of the ferrule is facing the screw. Pull the tubing
with pliers to ensure the bevel or taper is completely past the ferrule.
3. Hold the ferrule with a second set of pliers and give the tubing a half
twist to lock the ferrule. Cut the tubing flush with the end of the ferrule.
Figure 2: Ferrule and Compression Assembly (Non-Metallic Column)
c. Configuring the Column In the System
Install stainless steel or non-metallic anion columns relative to the
other major components as shown in Figure 3.
Figure 3: IC System for Anion Analysis
Install cation columns with a guard column for detecting monovalent
cations (Figure 4) or without a guard column for polyvalent cations
(Figure 5).
Figure 4: IC Systems for Monovalent Cation Analysis
Figure 5: IC Systems for Divalent Cation Analysis
III. eluent and sample guIdelInes
a. Preparing the Eluent Requirements
Water-miscible organic solvents, which may be used as modifiers to
reduce hydrophobic interaction between sample and packing, must
not exceed the concentrations listed below.
Note: Organic solvent concentration should not exceed 20%. It is
possible, however, to use organic solvents up to 50% concentration
by gradual introduction via gradient method. Perform solvent
changeovers at 0.1 mL/min over 6 hours. Dedicate column to use in
the organic concentrations over 20%.
Use high performance liquid chromatography (HPLC) grade solvents
that have been filtered to remove microparticulate matter greater
than 0.45 µm in size. Filtering will ensure optimum long-term
performance of the column. Vacuum filtration, sonication, or sparging
may be used to remove dissolved gas which could affect results.
Before filtering or clarifying any solvents, flush the filters with
100 mL of the eluent. Discard the filtered eluent. Surfactants used to
treat some filters may otherwise affect analysis.
An in-line filter may be used to remove particulates in the solvent
and contaminants which the 0.45 µm filter does not remove.
Note: Do not use tetrahydrofuran (THF) or methanol with IC-Pak A columns.
COMPRESSION SCREW
.021-inch (i.d.)
TEFZEL TUBING
TEFLON® FACE
BEVEL
FERRULE
STAINLESS STEEL
FACE
FROM SOLVENT
RESERVOIR
PUMP Inj
OPTIONAL
GUARD-PAK
AND HOLDER
IC-PAK
A
COLUMN
DETECTOR TO WASTE
HIGH SENSITIVITY
NOISE FILTER
PUMP Inj
HIGH SENSITIVITY
NOISE FILTER
ELUENT
EDTA/ HNO
3
GUARD COLUMN Inj IC-PAK C 430/431
PUMP Inj
HIGH SENSITIVITY
NOISE FILTER
ELUENT
EDA/ HNO
3
Inj IC-PAK C 430/431
IC-Pak A Columns 12% acetonitrile in water
IC-Pak C and Guard Columns 10% acetonitrile in water