Waters IC-Pak Column and Guard - Manual (Page 5)

[ Care and Use ManUal ]
IC-Pak Column and Guard Column 5
3. Fill to the mark with Milli-Q water and mix thoroughly. Concentrate
may be stored for up to six months before requiring replacement.
To prepare Sodium Borate/Gluconate Eluent, conductivity 270 RS:
1. Place approximately 500 mL of Milli-Q water into a one liter volumet-
ric flask. To this add:
20 mL borate gluconate concentrate 20 mL n-butanol
120 mL acetonitrile
2. Fill the flask to the mark with Milli-Q water and mix thoroughly. Filter
through a 0.22 µm GHP membrane.
d. Monovalent Cation Eluent
All reagents should be of the highest purity available.
1. To approximately 500 mL of Milli-Q water, add 120 ELL of concen-
trated nitric acid (Ultrex ® grade).
2. Accurately weigh 18.6 mg of disodium EDTA and add this to the nitric
acid solution.
3. Dilute the solution to one liter with Milli-Q water.
4. Filter particulate matter from the solution with one of the following Pall
Life Science membranes (refer to Section VI for part numbers):
Aqueous replacement filters, GHP (0.45 µm, 47 mm)
GHP filters (0.22 µm, 47 mm)
5. Degas the eluent.
e. Divalent Cation Eluent
All reagents should be of the highest purity available.
1. To prepare the stock HNO
3
solution, add 2.00 mL Ultrex HNO
3
(nitric
acid) to a 100 mL volumetric flask and dilute to the mark with Milli-Q
water. Store in a refrigerator for no longer than six months.
2. To a one liter volumetric flask filled with approximately 500 mL of
deionized water, add 35 µL anhydrous ethylene diamine (EDA).
3. Add 3.25 mL of stock HNO3 prepared in Step 1 above.
4. Fill to the mark with Milli-Q water and mix thoroughly.
5. Filter particulate matter from eluent with GHP filters, (0.45 µm, 47
mm, refer to Section VI for part number).
f. Sample Preparation and Filtration
Filter prepared samples using a GHP membrane filter to prevent
excessive pressure buildup due to particulate matter. Rinse the filter
with 10 mL of 18 megohm water then filter the first few drops of
sample through the filter to waste.
IV. operatIon
a. Chromatography Guidelines
Liquid chromatography columns have a finite life which is directly
related to the care and use they receive. Column life is reduced
by contamination from samples and solvents, frequent solvent
changeovers, and improper handling and storage.
If changes in the peak shape, retention of a particular compound,
or resolution between two compounds occurs, take immediate
steps to determine the reason for the changes. Until the cause is
determined, do not rely on the results of the analyses.
Follow generally accepted procedures for quality control and
methods development when using these columns.
Note: Before running the first analysis on the new column, perform
the test sample separation given in the Efficiency Testing section.
Precautions
Maximum pressure should not exceed:
Recommended Flow Rate:
pH Range for all columns: 1-12
IC-Pak A, IC-Pak C and Guard Column 6.7 MPa (67 atm or 1000 psi)
IC-Pak A HR 13 MPa (130 atm or 2000 psi)
IC-Pak A HC 20 MPa (200 atm or 3000 psi)
IC-Pak A 1.2 mL/min
IC-Pak A HC 2.0 mL/min*
IC-Pak A HR 1.0 mL/min
IC-Pak C 1.2 mL/min