Waters HILIC QC Reference Material - Manual

[ CARE AND USE MANUAL ]
HILIC Quality Control Reference Material
CONT ENTS
I. INTRODUCTION
II. STORAGE AND STABILITY
III. EXAMPLE OF USING THE HILIC QCRM
IV. ORDERING INFORMATION
I. INTRODUCTION
The Quality Control Reference Material (QCRM) portfolio is a unique
collection of standards and mixtures. These products allow the user to
evaluate and benchmark their chromatography system before analysis
of critical material. The products in the portfolio are all specifically
formulated based on the expertise of Waters scientist.
The retention of polar analytes and metabolites is a chromatographic
challenge for scientists. Polar compounds often elute in the void of most
reversed-phase columns or end up co-eluting with endogenous species
present within various sample matrices in RPLC. Hydrophilic interaction
chromatography (HILIC) is a complementary technique to reversed phase
and normal phase, that can be used to successfully improve the retention
of very polar species.
1
The HILIC QCRM was designed as a standard to help benchmark this type
of separation. It is a four component mix containing an un-retained void
marker, a polar neutral compound, and two polar basic compounds that
provides a stable chromatographic separation for HILIC columns.
HILIC QCRM Components
Compound Name Property Concentration in Vial (mg/mL)
Acenaphthene V0 marker 0.0190
Thymine Polar neutral 0.0037
Adenine Polar basic 0.0037
Cytosine Polar basic 0.0077
Figure 1. Individual Compound Formulas.
acenaphthene cytosine thymine adenine