Waters MassPREP Online Desalting Cartridge - Manual

CONT ENTS
I. INT RODUCTION
II. INSTALLING T HE MASSP REP ON-LINE
DESALTING CART RIDGE INTO T HE SENT RY
2.1 X 10 MM GUARD COLUMN HOLDER
III. RECOMMENDED LC/MS SYSTEM CONFIGURATION
TO MINIMIZE MS SOURC E CONTAMINATION
IV. SUGGEST ED MET HOD FOR MASS ANALYSIS
USING MASSP REP ON-LINE DESALTING
CART RIDGE
a. Instrumentation
b. Separation Method
V. GUIDELINES FOR USE AND
T ROUBLESHOOTING
VI. MISC ELLANEOUS
I. INT RODUCTION
Mass spectrometry is a powerful tool for intact protein analysis.
However, the quality of analyses can be compromised by salts in
the sample that interfere with ionization and/or form adducts with
the investigated proteins. It is therefore important to remove or
significantly minimize the introduction of extraneous salt ions
into the mass analyzer. The use of on-line, desalting devices can
efficiently perform this function with minimal sample handling.
Waters MassPREP™ On-line Desalting Cartridges (2.1 x 10 mm,
P/N 186002785) contain a polymer-based, reversed-phase
packing material that can be used to effectively desalt protein
samples prior to mass spectrometry. (Note: The purchase and use
of Waters Sentry™ 2.1 x 10 mm Guard Column Holder Kit (P/N
WAT097958) is required for this application as shown in Figure 1.)
The phenyl phase material contained in these on-line devices
successfully “traps” proteins allowing the salts to be washed
to waste prior to elution of the desalted protein into the mass
spectrometer. With an optimized LC/MS method, cycle times as low
as 3 minutes are achievable. These on-line devices, when used with
an appropriate HPLC System injector and injector wash solution,
can be used for the sequential analyses of different samples (See
Figures 2 and 3).
This Care and Use manual provides suggested methods for the
successfully use of this product. For the latest list of MassPREP,
BioSuite™ or NanoEase™ column offerings, go to www.waters.com/
lifesciences .
II. INSTALLING T HE MASSP REP ON-LINE
DESALTING CART RIDGE INTO T HE SENT RY
2.1 X 10 MM GUARD COLUMN HOLDER
1. Unscrew the two halves of the Sentry 2.1 x 10 mm Guard
Column Holder.
2. Remove the old MassPREP On-line Desalting Cartridge, if
present.
MassPREP On-Line Desalting Cartridge
[ CARE AND USE MANUAL ]