Waters AccQ-Tag Ultra Derivatization Kit - Manual

[ CARE AND USE MANUAL ]
AccQ•Tag Ultra Derivatization Kit
CONT ENTS
I. INTRODUCTION
II. ACCQ•TAG ULTRA OVERVIEW
III. CONTENTS OF KIT
IV. SAMPLE PREPARATION
V. STORAGE AND STABILITY
I. INTRODUCTION
The AccQ•Tag™ Ultra Derivatization Kit is a component of the UPLC ®
Amino Acid Analysis Application Solution. This application solution is
an integrated combination of instrumentation, derivatization chemistry,
separation column and eluents, methods, and software. Analysts are
assured of accurate and precise amino acid analyses with the complete
application solution. The use of the AccQ•Tag Ultra Derivatization Kit
without the rest of the application solution is not supported as an amino
acid analysis method because there are significant risks of inaccurate
qualitative and quantitative analysis.
The AccQ•Tag Ultra Derivatization Kit differs from the AccQ•Tag
Derivatization Kit (P/N WAT052880). Although the specified chemical
composition is the same for both kits, the quality control tests for The
AccQ•Tag Ultra Derivatization Kit are executed with UV detection. In
contrast, the quality control tests for The AccQ•Tag Derivatization Kit are
executed with fluorescence detection. Substituting one kit for the other
may result in unexpected interferences that may compromise sensitivity or
introduce ambiguity into peak identification.
II. ACCQ•TAG ULTRA OVERVIEW
The UPLC Amino Acid Analysis Application Solution is pre-column
derivatization and analysis technique. To perform this analysis:
1. Use AccQ•Tag Ultra Reagent Powder (US Patent 5,296,599 and
European Patent EP 0 533 200 B1) to derivatize the amino acids.
2. Separate the derivatives using the properly configured ACQUITY
UPLC System.
3. The reversed phase separation occurs on the Waters AccQ•Tag Ultra,
2.1 x 100 mm, 1.7 μm column. The derivatives are eluted with the
specified dilution of Waters AccQ•Tag Ultra Eluent A and Waters
AccQ•Tag Ultra Eluent B.
4. Quantitate the derivatives with UV detection.
5. Process the data using Empower ® software.
For complete details of the instrument configuration and of the separation
and analysis methods, consult the UPLC Amino Acid Analysis Application
Solution System Guide.