Waters ACQUITY UPC2 Torus Columns User Manual
Acquity upc, Torus columns, Care and use manual
[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
II. GET TING START ED
a. Safety Considerations
b. Column Connectors and Installation
c. eCord Installation
d. Column Equilibration
e. Initial Column Efficiency Determination
f. VanGuard Pre-Columns
III. COLUMN USE
a. Sample Preparation
b. Solvents
c. Additives
d. Pressure
e. Useable Flow-Rate Ranges and Expected ACQUITY UPC
2
System Pressures
f. Band Spreading Minimization
IV. T ROUBLESHOOTING
V. COLUMN CLEANING, ADDITIV ES,
AND STORAGE
a. Cleaning
b. Effects of Additives
c. Aging
d. Storage
VI. INT RODUCING ECORD INT ELLIGENT
CHIP T ECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
VII. ACQUIT Y UPC
2
COLUMN FAMILY
ACQUITY UPC
2
Torus Columns
I. INT RODUCTION
Thank you for choosing an ACQUITY UPC
2
®
Torus™ Column.
Torus Columns are specifically designed to use the complete
range of capabilities of the ACQUITY UPC
2
System to achieve fast,
robust achiral separations. ACQUITY UPC
2
Torus Columns simplify
the method development process with four completely new and
innovative 1.7 µm chemistries for UltraPerformance Convergence
Chromatography.™ These columns are designed for excellent
peak shape that eliminate or reduce need for additives, coupled
with added selectivity for a wide range of compounds and
improved robustness.
These columns are based on new bonding technology used to
create an adaptive selectivity platform. Starting with the ethylene
bridged hybrid (BEH) particle, the patent pending bonding consists
of a two stage functionalization of chromatographic particles.
The first stage controls retention characteristics and is applied
to each sorbent. The individual second stage selectors impart
the observed selectivity and control peak shape.