Waters ACQUITY UPC2 Trefoil Columns - Manual

[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
II. GET TING START ED
a. Safety Considerations
b. Column Connectors and Installation
c. eCord Installation
d. Column Equilibration
e. Initial Column Efficiency Determination
III. COLUMN USE
a. Sample Preparation
b. Solvents
c. Additives
d. Pressure
e. Band Spreading Minimization
IV. T ROUBLESHOOTING
V. COLUMN CLEANING, ADDITIV ES,
AND STORAGE
a. Cleaning
b. Effects of Additives
c. Storage
VI. INT RODUCING ECORD INT ELLIGENT
CHIP T ECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
VII. ACQUIT Y UPC 2 COLUMN FAMILY
ACQUITY UPC 2 Trefoil Columns
I. INT RODUCTION
Thank you for choosing an ACQUITY UPC 2 ® Trefoil™ Column.
ACQUITY UPC 2 Trefoil Columns are specifically designed to use
the complete range of capabilities of the ACQUITY UPC 2 System
to achieve fast, robust chiral separations. ACQUITY UPC 2 Trefoil
Columns are uniquely designed for the ACQUITY UPC 2 System
to enable both selectivity and speed in chiral separations and to
reduce method development time. ACQUITY UPC
2 Trefoil Columns
consist of modified polysaccharide coated stationary phases for
broad-spectrum chiral selectivity. The higher order structure of
the polysaccharides have specific spatial orientations that impart
chiral selectivity through well-chosen modifications or selectors.
ACQUITY UPC 2 Trefoil AMY1, CEL1, and CEL2 column
chemistries are complementary to each other and independently
offer different retention characteristics for separating chiral
compounds. Selectivity can be further enhanced by using
co-solvents and additive blends that most favorably modulate
chiral recognition. These columns are designed to separate
enantiomers, stereoisomers, metabolites, degradants, and
impurities with greater resolution and speed.