Waters Oasis HLB Cartridges and 96-Well Plates User Manual

Oasis hlb cartridges and 96-well plates, Care and use manual

[ CARE AND USE MANUAL ]

CONT ENTS

I. INT RODUCTION

II. SAMPLE P RE-T REATMENT

a. Biological Samples

b. Solid Samples: Soil, Whole Foods, Tissue

c. Aqueous Samples: Water, Beverages

d. Non-Aqueous Liquid

III. SOLID PHASE EX T RACTION FOR ACIDIC,

NEUT RAL, AND BASIC COMPOUNDS USING

OASIS HLB

IV. OASIS HLB P ROTOCOL CHARTS

a. Generic Method Oasis HLB SPE

b. Strategy for Optimizing the Generic SPE Method

V. OASIS HLB 20 BOT T LE

OPTIMIZATION AP P ROACH

VI. T ROUBLESHOOTING

a. Adjustment to Optimize Recoveries

Oasis HLB Cartridges and 96-Well Plates

I. OASIS HLB

Universal Sorbent for Acidic, Neutral, and Basic Compounds
Oasis

HLB is a Hydrophilic-Lipophilic-Balanced, water-wettable,

reversed-phase sorbent for all your SPE needs. It is made from a
specific ratio of two monomers, the hydrophilic N-vinylpyrrolidone
and the lipophilic divinylbenzene. It provides superior reversed-
phase capacity with a neutral polar ‘hook’ for enhanced retention of
polar analytes.

Waters

has built a family of SPE sorbents which inherit some

key features of this unique substrate: stability at pH extremes
and in a wide range of solvents, extraordinary retention of polar
compounds, and a relative hydrophobic retention capacity 3X
higher than that of traditional silica-based SPE sorbents like C

Water-wettable Oasis Sorbents exhibit excellent retention capacity
for a wider polarity spectrum of analytes, even if the sorbent bed
runs dry during conditioning or sample loading. This means that
your SPE methods will be more rugged and robust, obviating the
need for repeat preparation.

The advantage of having higher retention capacity [k] is that
more analytes are retained with less breakthrough, improving the
recovery and overall reproducibility of your SPE method.

Available in five particle sizes [60 μm, 30 μm, 25 μm, 15 μm, and
5 μm], Oasis HLB sorbent, in cartridge, plate, or column format,
allows you to select the appropriate product based on the volume,
viscosity, and turbidity of your sample.