Waters Oligonucleotide Separation Technology XBridge OST C18 Columns User Manual
Method guidelines, Xbridge, Ost c
[ method guidelines ]
XBridge
™
OST C
18
columns are based on Waters second generation of
hybrid-silica BEH Technology
™
particles and can be effectively used
for the lab scale purification and analysis of detritylated synthetic
oligonucleotides using ion-pair, reversed-phase chromatography. This
document provides useful method guidelines for the effective use of
this column chemistry for this group of compounds.
Contents
I.
PRINCIPLES OF OLIGONUCLEOTIDE SEPARATIONS
II. SAMPLE PREPARATION
III. RECOMMENDED MOBILE PHASES
IV. RECOMMENDED INJECTOR WASH SOLVENT
V. GENERAL CONSIDERATIONS IN DEVELOPING SEPARATIONS
VI. ANALYSIS OF MODIFIED OLIGONUCLEOTIDES
VII. PURIFICATION CONSIDERATIONS
XBridge
™
ost C
18
Method guidelines
*ost: oligonuCleotide separation teChnology
- Protein-Pak Hi Res HIC Column and HIC Protein Standard (5 pages)
- ACQUITY UPC2 Trefoil Columns (7 pages)
- ACQUITY UPC2 Torus Columns (8 pages)
- XBridge Protein BEH SEC Columns and Standards (6 pages)
- ACQUITY UPLC M-Class Columns (9 pages)
- AccQ-Tag Ultra C18 Column (3 pages)
- Reverse Peptide Kit (4 pages)
- Enzymate BEH Pepsin Columns (5 pages)
- Oasis Mixed-Mode Ion-Exchange Cartridges and 96-Well Plates (6 pages)
- Oasis HLB Cartridges and 96-Well Plates (6 pages)
- Sep-Pak XPoSure Aldehyde Sampler (11 pages)
- CORTECS 2.7 um Columns (8 pages)
- MassPREP Online Desalting Cartridge (5 pages)
- MassPREP Micro Desalting Column (3 pages)
- AccQ-Tag Ultra Derivatization Kit (3 pages)
- Quantitative Peptide Retention Standard (3 pages)
- MassPREP Peptide Mix Standard (2 pages)
- Intact mAb Mass Check Standard (3 pages)
- HILIC QC Reference Material (3 pages)
- SILAC Hi3 Standards (4 pages)
- MetID Small Molecule Standard Mix (3 pages)
- Beverage Analysis Kit (3 pages)
- iKey (11 pages)
- Forensic Toxicology Installation Standards Kit (3 pages)
- XBridge BEH Glycan, 2.5 μm XP and 3.5 μm Columns and Standards (6 pages)
- nanoACQUITY UPLC 2G Trap Columns (3 pages)
- Biopharmaceutical Training Kit (5 pages)
- Protein-Pak Hi Res IEX Columns (9 pages)
- LCMS Quality Control Reference Materials (3 pages)
- Dye QCRM Kit (8 pages)
- GlycoWorks High-throughput Sample Preparation Kit (6 pages)
- GlycoWorks Single Use Sample Preparation Kit (6 pages)
- 30 mm ID Preparative Guard Cartridges and Guard Holders (5 pages)
- ACQUITY APC Columns (7 pages)
- Soft Drink Analysis Kit (3 pages)
- Certified Containers (3 pages)
- CORTECS 1.6 um Columns (9 pages)
- Extraction Manifold (6 pages)
- Protein-Pak SEC Columns (6 pages)
- Neutrals Quality Control Reference Materials (18 pages)
- Preparative Chromatography Mix Standard (7 pages)
- Dextran Calibration Ladder Standard (3 pages)
- Oligonucleotide Separation Technology XBridge OST C18 Columns (7 pages)
- AccellPlus QMA and CM Bulk Media (5 pages)