Waters CORTECS 2.7 um Columns - Manual

CONT ENTS
I. INT RODUCTION
II. GET TING START ED
a. Column Connection
b. Column Installation
c. Minimizing Band Spread Volume
d. Measuring Band Spread Volume
e. Measuring System Dwell Volume
f. Column Equilibration
g. eCord™ Installation
h. Functional Tests for Benchmarking a New Column
i. VanGuard™ Pre-Columns
III. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
IV. COLUMN CLEANING, REGENERATION
AND STORAGE
a. Cleaning and Regeneration
b. Storage for Reversed-Phase and HILIC columns
V. ECORD INT ELLIGENT CHIP T ECHNOLOGY
a. Introduction
b. Installation
c. Column Use Information
VI. ADDITIONAL INFORMATION
a. Tips for Maximizing Column Lifetime
b. Getting Started with CORTECS ® HILIC Columns
c. Troubleshooting Questions
I. INT RODUCTION
Thank you for choosing a Waters CORTECS Column. CORTECS 2.7 µm
Columns are based on the same solid-core particle technology
as CORTECS UPLC
® 1.6 µm Columns, enabling seamless method
transfer between HPLC, UHPLC and UPLC platforms. All CORTECS
Columns are manufactured in cGMP, ISO 9001:2000 certified
facilities. Strict quality procedures for the manufacture of
the solid-core particle, the bonded phase synthesis, and the
column packing result in a high quality and reproducible final
product. Every column is individually tested and a Performance
Chromatogram and Certificate of Batch Analysis are provided on
the eCord Intelligent Chip. The eCord is available on all 2.1 mm
and 3.0 mm column dimensions.
CORTECS 2.7 µm Columns will exhibit maximum chromatographic
performance when used on a member of the ACQUITY UPLC
®
System family.
CORTECS 2.7 µm Columns