Waters Dextran Calibration Ladder Standard - Manual

[ CARE AND USE MANUAL ]
Dextran Calibration Ladder Standard 1
I. INTRODUCTION
Hydrophilic Interaction Liquid Chromatography (HILIC) coupled
with Fluorescent Detection is widely used for fluorescent (FLR)
labeled glycans. Waters ACQUITY
® BEH Glycan column operated
in HILIC mode shows drastic improvement in separation e.g., peak
resolution and the ability to separate both neutral and acidic glycans,
compared to the conventional HPLC methods in HILIC mode (Ref. 1).
The ACQUITY BEH Glycan column enables scientists to get robust
and reproducible glycan separation data with less time spent in
method optimization.
In order to take full advantage of the BEH glycan column, Waters
created the 2-aminobenzamide labeled Dextran Calibration Ladder
Standard to assist glycan profiling: glycan profile obtained from
UPLC-HILIC/FLR system can be calibrated against the 2AB-labeled
Dextran ladder and assigned with glucose unit (GU) values (Ref.2).
Waters 2AB-labeled Dextran Calibration Ladder is different than
the existing commercial offerings. The average molecular weight of
the glucose homopolymer is higher (~4,500 Dalton), therefore,
the “workable” GU value range is twice as much as the other
Dextran ladder standards; the observed GU goes from 2 to 30.
This feature improves the large glycan retention time assignment.
The purity and structural integrity of the ladder is assessed by
normal phase HPLC and MS.
II. STORAGE AND STABILITY
The lyophilized powder is shipped at ambient temperatures, but it
is highly recommended upon receipt of the standard to store it
refrigerated (4°C) until the expiration date printed on the label. Once
reconstituted in should be used immediately for best results.
III. RECONSTITUTION OF THE DEXTRAN CALIBRATION LADDER
One vial of the Dextran Calibration Standard contains 200 µg of a
lyophilized, solid powder. It is contained in a Waters Max Recovery Vial
for supreme ease of use so that it can be directly solubilized and put on
the system for analysis and injection.
Dextran Calibration Ladder Standard
CONTENTS
I. INTRODUCTION
II. STORAGE & STABILITY
III. RECONSTITUTION OF DEXTRAN CALIBRATION LADDER
IV. BENEFITS OF USING A CALIBRATION STANDARD
V. EXAMPLES OF USING THE DEXTRAN CALIBRATION
LADDER ON THE ACQUITY UPLC
VI. ORDERING INFORMATION