Waters Protein-Pak Hi Res IEX Columns - Manual

[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
II. CONFIGURING AN ACQUIT Y SYST EM FOR
USE IN IEX P ROT EIN SEPARATIONS
III. GET TING START ED
a. eCord Installation
b. Column Connectors
c. Column Installation
d. Column Equilibration
e. Useful Functional Tests for
Benchmarking a New Column
IV. COLUMN SPECIFICATIONS AND USE
a. Specifications
b. Sample Preparation
c. Operating pH
d. Solvents
e. Pressure
f. Flow Rate
g. Temperature
V. T ROUBLESHOOTING
VI. COLUMN CLEANING, REGENERATION,
AND STORAGE
a. Cleaning and Regeneration
b. Storage
VI. INT RODUCING ECORD INT ELLIGENT
CHIP T ECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
VIII. ORDERING INFORMATION
Protein-Pak Hi Res IEX Columns and Standards
I. INT RODUCTION
Thank you for choosing a Protein-Pak™ ion-exchange column.
Waters Protein-Pak Hi Res IEX family of columns were developed
to assist in the UPLC
® characterization of recombinant proteins
and monoclonal antibodies found in many of today’s novel
biopharmaceutical therapeutics. The non-porous, high-ligand
density particles overcome some of the high molecular weight
limitations present using traditional porous, IEX particles. In
addition, use of a multi-layered network of ion-exchange groups
(SP, CM, or Q) on non-porous particles assist the effective diffusion
and binding of charged proteins or other biomolecules onto the
particles to deliver relatively high sample loading capacities and
component resolution while minimizing column fouling.
The available ion-exchange chemistries include a strong-anion
and weak- and strong-cation exchangers (Table 1), all of which are
stable, over a wide pH range (3–10), high salt concentrations and
standard pressures (see Column Specifications and Use section) via
use of polymer-based particles. Only when the Protein-Pak Hi Res
IEX column series is combined with the ACQUITY UPLC
® system
will the full performance benefits of this column be realized.
Table 1
Abbreviation Ion-Exchange Ligand
SP Sulfopropyl
CM Carboxymethyl
Q Quaternary ammonium
columns