Waters MassPREP Peptide Mix Standard - Manual

[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
II. STORAGE AND STABILIT Y
III. RECONSTITUTION OF MASSP REP
PEPTIDE MIX TURE
IV. MSDS INFORMATION
V. ORDERING INFORMATION
MassPREP Peptide Mixture
INT RODUCTION
The peptide mixture contains nine peptides with a broad range of
polarities, and isoelectric points (pI). In addition, there is a void
volume marker (Table 1). All peaks are resolved under standard
reversed-phase LC conditions typically used for peptide separation
(Figure 1). Therefore the mixture can be used for the evaluation of
columns and LC systems dedicated for peptide separations.
Peak #
Component
Name
Retention Time
*(minutes)
Molecular Weight
**(g/mol)
pKa Peptide Sequence
1 Allantoin (V0 marker) 0.76 158.0440 - -
2 RASG-1 4.78 1000.4938 9.34 RGDSPASSKP
3 Angiotensin frag.1-7 9.59 898.4661 7.35 DRVYIHP
4 Bradykinin 12.04 1059.5613 12.00 RPPGFSPFR
5 Angiotensin II. 13.06 1045.5345 7.35 DRVYIHPF
6 Angiotensin I. 14.92 1295.6775 7.51 DRVYIHPFHL
7 Renin substrate 16.82 1757.9253 7.61 DRVYIHPFHLLVYS
8 Enolase T35 19.18 1871.9604 7.34 WLTGPQLADLYHSLMK
9 Enolase T37 21.55 2827.2806 3.97 YPIVSIEDPFAEDDWEAWSHFFK
10 Melittin 24.93 2845.7381 12.06 GIGAVLKVLTTGLPALISWIKRKRQQ
Table 1
* BioSuite™ C18 3.5 µm PA-B Column (4.6 x 50 mm), mobile phase A: 0.1% TFA
in water, B: 0.08% TFA in acetonitrile, gradient: 0-50% B in 30 minutes, 0.96
ml/min, 40° C 214 nm. Retention times will change if different LC conditions,
column, and LC system are used.
** Monoisotopic molecular weight, MassLynx™ v. 4.0
18
3.5 µm PA-B
AU
0.0
0.5
Minutes
0 10 20 30
Allantoin
RASG-1
Angiotensin frag. 1-
7
Bradykinin
Renin substrate
Angiotensin II Angiotensin
I
Enolase T37
Melittin
Enolase T35
For chromatographic conditions see Table 1.
Figure 1: Standard Peptide Mixture on BioSuite C18 3.5 µm PA-B Column
(4.6 x 50 mm).