Waters SILAC Hi3 Standards - Manual

[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
II. STORAGE AND STABILIT Y
III. RECONSTITUTION OF T HE
SILAC HI3 DIGESTION STANDARDS
IV. USING T HE SILAC HI3 DIGESTION
STANDARDS FOR QUANTIFICATION
V. ORDERING INFORMATION
SILAC Hi3 Standards
I. INT RODUCTION
The Hi3 stable isotope labeled (SIL) standards (Phos B and ClpB)
provides an isotopically unique exogenous standard to perform
Hi3 relative protein quantitation.
■ ■
Minimize interferences with the endogenous peptides
■ ■
Perform relative protein quantitation of mixed proteome samples
■ ■
Add a second level of internal quality control (i.e., spike
Hi3 SILAC standards toward the lower limit of detection).
These standards, along with their unlabeled counterparts, are
quantitated via AAA to equimolar ratios to provide greater accuracy to
each analysis. They are greater than 95% pure and have been verified
by mass identification. In addition, each vial is in a Waters Maximum
Recovery Vial which simplifies the sample preparation process.
These standards are primarily intended for use with the Hi3
quantification method for MS
E proteomics data processed with
ProteinLynx GLOBAL SERVER.™ The E. coli standard is intended for
samples of animal origin, and the Phos B standard is intended for
samples of microbial origin. The standards may also be of use in the
evaluation and benchmarking of proteomic LC/MS systems comprised
of nanoACQUITY UPLC
® and SYNAPT ® and Xevo ® time-of-flight
mass spectrometers.