Waters Reverse Peptide Kit - Manual

CONT ENTS
I. INT RODUCTION
a. Parts Required
b. Additional Materials Required But Not Supplied
II. RECOMMENDED SAMPLE
P REPARATION P ROC EDURES
a. Sample Preparation Guidelines
b. Health and Safety Precautions
c. Storage and Handling
III. REV ERSE PEPTIDE STANDARD
P REPARATION P ROTOCOL
a. Diluent
b. Reverse Peptide Stock Solutions
c. Reverse Peptide Standard Solution Working Concentration
IV. MOBILIT Y MODE COMMISSIONING T EST
a. Resolution Mode (Positive Ion)
b. Saving System Settings
V. ORDERING INFORMATION
Reverse Peptide Kit
I. INT RODUCTION
The Reverse Peptide Kit (P/N 700005089) contains two well-
characterized samples (Ser-Asp-Gly-Arg-Gly and Gly-Arg-Gly-Asp-Ser)
used to calibrate ion mobility spectrometry/mass spectrometry
(IMS/MS) systems in high-resolution positive-ion mode. Analysts
can also use the reverse peptide standard solution prepared
from these samples as a reference standard for verifying IMS
performance.
The instructions in this manual describe the recommended
procedure for preparing the reverse peptide standard solution and
performing the mobility mode commissioning test.
a. Parts Required
Table 1: Kit components
Serial Sample
Supply
format
Concentration/
weight
Number of
vials
1
Ser-Asp-
Gly-Arg-Gly
Powder
1 mg
(–0 +0.2 mg)
1
2
Gly-Arg-
Gly-Asp-Ser
Powder
1 mg
(–0 +0.2 mg)
1
b. Additional Materials Required But Not Supplied
Table 2: Additional materials
Solvents Additives Labware
Acetonitrile
(Fisher Scientific
high-purity or
equivalent-
quality solvents) >98% purity formic
acid (Aristar-
grade additives;
avoid GPR-grade
additives) 20-mL Class A
volumetric flasks (2)
30-mL Nalgene
container (w/cap)
Water
(ELGA or
equivalent-
quality
(18 MΩ purity)
lab water)
100-mL graduated
measuring cylinder
250-mL Duran laboratory
glass bottle (w/cap)
200-μL pipette (and tip)
(Gilson or equivalent-
quality pipettes)
1000-μL pipette (and tip)
[ CARE AND USE MANUAL ]