Waters ACQUITY APC Columns - Manual

ACQUITY APC Columns
CONT ENTS
I. INTRODUCTION
II. GETTING STARTED
a. Preparing the System
b. Column Connections
c. Column Installations
d. eCord Installation
e. Column Equilibration
III. COLUMN USE
a. Guidelines
b. Calibration
c. Useable Flow-Rate Ranges
d. Minimizing Band Spread
IV. TROUBLESHOOTING
V. COLUMN CLEANING, REGENERATION
AND STORAGE
a. Cleaning and Regeneration
b. Storage
VI. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Column Use Information
I. INTRODUCTION
Thank you for choosing an ACQUITY APC™ Column. The ACQUITY APC
Columns are designed to achieve maximum separation performance
when used with the ACQUITY
® Advanced Polymer Chromatography ®
(APC™) System. Please take a few moments to read this manual carefully.
Following the recommendations in this manual will prolong column life
and enhance chromatographic reproducibility.
ACQUITY APC Columns are packed with sub-3-µm ethylene bridged
hybrid (BEH) particles that provide mechanical strength, packed-
bed stability and high separation efficiency. They deliver superior
chromatographic performance for all polymer classes, including low
molecular weight aqueous and organic soluble polymers, up to a
molecular weight of 2,000,000 g/mole (Table 1).
To maximize separation selectivity and performance ACQUITY APC
Columns are available in two bonding technologies optimized for each
solvent class:
ACQUITY APC XT: Packed with a high-coverage trimethyl silane bonded to
a BEH substrate. These columns are recommended for extended temperature
(< 90 °C) separations in organic solvents.
ACQUITY APC AQ: Feature unbonded BEH substrate. Recommended for
room temperature (< 45 °C) separations in aqueous solvents.
ACQUITY APC Columns are manufactured in an ISO 9001 facility and are
held to narrow specification ranges to ensure reproducible performance.
Every column is individually tested and a Certificate of Batch Analysis is
provided on the attached eCord™ Intelligent Chip.