Waters XBridge Protein BEH SEC Columns and Standards - Manual

[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
II. SYST EM CONSIDERATIONS FOR
SEC SEPARATIONS
a. Getting Started
b. Column Installation
c. Column Equilibration
d. Useful Functional Tests for Benchmarking LC System
and XBridge Protein BEH SEC Column
III. COLUMN SPECIFICATIONS AND USE
a. SEC Eluent and Needle Wash Preparation
b. Sample Preparation
c. Column Specification
IV. T ROUBLESHOOTING
V. COLUMN CLEANING, REGENERATION
AND STORAGE
a. Cleaning and Regeneration
b. Storage
XBridge Protein BEH SEC Columns and Standards
I. INT RODUCTION
Waters family of XBridge ® Protein BEH SEC 200Å and 450Å,
3.5 µm Columns was developed to complement the existing line of
UPLC
® -based SEC offerings for use where traditional HPLC-based
instrumentation and methods are employed for peptide or protein
size-exclusion chromatography (SEC). These new HPLC-based, SEC
chemistries are based on the same Waters Ethylene Bridged Hybrid
(BEH)-based particle technology and diol-bonded surface coating
as used in our successful line of UPLC-based SEC columns. This
process offers chromatographers the option and ability to easily
transfer methods based on lab instrumentation and component
resolution or sample throughput needs.
All of Waters BEH-based SEC columns are manufactured in a cGMP,
ISO 9001 certified plant using stringent manufacturing protocols
and ultra pure reagents. Each batch of manufactured material
undergoes a series of standard QC measurements (e.g., particle
and pore size distribution) followed by an application specific
test using appropriate peptide and protein test mixture. A packed
column efficiency test is then performed on every batch approved,
packed SEC column to further help ensure reproducible batch to
batch and column to column performance for use in research or in a
demanding validated method.