Waters Oligonucleotide Separation Technology XBridge OST C18 Columns - Manual

[ CARE AND USE MANUAL ]
CONTENTS
I. GETTING STARTED
a. Connecting the Column
b. Column Equilibration
c. Initial Column Efficiency Determination
II. COLUMN USE
a. Sample Preparation
b. Recommended Mobile Phases
c. Recommended Injector Wash Solvents
d. pH Range
e. Pressure
f. Temperature
g. Flow Rate
III. COLUMN CLEANING, REGENERATING AND STORAGE
a. Cleaning and Regeneration
b. Storage
VI. ADDITIONAL INFORMATION
a. Band Spreading Minimization
b. Measuring System Bandspreading Volume and System Variance
c. Impact of Bandspreading Volume on 2.1 mm i.d. Column Performance
Thank you for choosing a Waters XBridge ™ OST C
18
column designed
for lab scale purifications and analyses of synthetic oligonucleotides.
The separation of detritylated oligonucleotides on Waters second gen-
eration of hybrid-silica BEH Technology
™ particles is based on the well
established method on ion-pair, reversed-phase chromatography. The
availability of XBridge OST C
18
material contained in several column
configurations provides flexibility to meet different application needs.
Your XBridge OST C
18
packing material is manufactured in a cGMP,
ISO 9002 certified plant using ultra pure reagents. Each batch
of XBridge OST C
18
material is chromatographically tested with
acidic, basic and neutral analytes and the results are held to narrow
specification ranges to assure excellent, reproducible performance.
In addition, every column is individually tested and the associated
Performance Test Chromatogram and Certificate of Acceptance is
included with your purchased column.
XBRIDGE ™ OST C
18
*OST: OLIGONUCLEOTIDE SEPARATION TECHNOLOGY