Waters Advanced Purification Glass Columns - Manual (Page 7)

[ Care and Use ManUal ]
Waters Advanced Purification Columns 7
3. Replace the set screw. Do not overtighten the set screw; the plunger
core must slide easily to permit the O-ring seal to form.
4. Reassemble the column.
d. Replacing End Fittings
The end fittings for the AP column are compression screws with
gripper rings, washers, and ferrules.
Making a new compression screw assembly
To make a new compression screw assembly:
1. Use a single-edge razor to make a straight, square cut on the plastic
tubing. Make the cut in front of the compression screw.
2. Slide a compression screw fitting and a washer over the end of the cut
tubing.
3. Place a gripper ring in the top depression of the tool (see Figure 5).
Insert the end of the tubing into the gripper ring tool as for as it will go.
This moves the gripper ring to the proper distance from the end of the
tubing.
4. Remove the tubing from the tool and slide the other washer and ferrule
over the end of the tubing.
5. Insert a union over the end fitting assembly and tighten it to seat the
end-fitting.
e. Replacing Filters and O-rings
The inlet and outlet connector assemblies each contain a filter.
Replace filters periodically, depending on:
•
The cleanliness of the sample and solvents
• The type and particle size of the packing material
Distributors for AP filters are inside the filter skirt. Look for the star-
like pattern on the distributor surface to ensure the distributor has
not become dislodged.
The AP column contains three O-rings, one each at the connectors
and one at the filter. The outlet connector O-ring does not normally
need replacement.
Accessing the filters
To access the inlet filter:
1. Deactivate the inlet O-ring by turning the seal adjustment knob
counterclockwise.
2. Slide the inlet connector adjustment to the coarse adjustment position,
and unscrew the plunger from the column.
To access the outlet filter, unscrew the outlet connector from the plastic
shield and remove from the glass column.
Installing the filter
To install on inlet filter:
1. Remove the old filter. Pry off the filter with a fingernail (or by inserting
a blunt flat tool between the filter and shoulder of the connector tip).
2. Ensure the distributor is in place inside the filter skirt of the new Filter.
3. Snap the new filter onto the connector tip
Figure 6: Inlet Connector Tip
Filter Filter Skirt Filter O-ring Plunger
Distributor Connector tip Connector O-ring