Waters Advanced Purification Glass Columns - Manual (Page 2)

[ Care and Use ManUal ]
Waters Advanced Purification Columns 2
a. AP Column Accessories
The following accessories are shipped with the AP column:
•
Packing funnel
• End plugs for column storage
• Spare Parts Kit consisting of:
• 1 inlet O-ring
• 1 outlet O-ring
• 1 connector tip O-ring
• 1 packing funnel O-ring
• 2 filter assemblies
• 2 ferrules
• 10 gripper ring tools
• 1 Allen wrench
Table 1: AP Column Specifications
II. usIng the Column
a. Safety and Solvent Considerations
Inherent surface imperfections can weaken glass at high pressures.
Maintain lower pressures to prolong column life. The AP glass
column has an external plastic jacket which acts as a safety shield.
To avoid column hardware damage, never exceed 10 MPa (1500 psi
or 100 atm) inside the column.
High backpressures may damage resin-based packing materials.
Consult your packing material care and use manual for packing
material backpressure limits.
AP column materials are compatible with most solvents used in
HPLC. However:
•
Certain solvents, such as acetone or chlorinated hydrocarbons,
may reduce the life expectancy of the O-rings.
• The transparent plastic jacket may weaken if exposed to certain organic
solvents. If the jacket crazes, replace it.
b. Adjusting the Plunger
Range of movement
The packed bed is at maximum volume when the hex at the inlet end
of the plunger is flush with the top of the coarse/fine adjustment
slide. Do not withdraw the plunger further, or too few threads will be
engaged to ensure reliable operation.
The slide must be engaged against the locking hex while the column
is in use to prevent the plunger from unscrewing under pressure.
Figure 2: Column at Maximum Volume
Internal diameter 10 mm
Length (nominal) 100, 200, 300, or 600 mm
Maximum operating pressure 10 MPa (100 Atm, 1500 psi)
Filter size 20 µm
Materials along fluid path:
Column
Tubing
Filter
Connectors
O-rings
Borosilicate glass
Tefzel
®
Polyethylene
Ryton-4
® (polyphenylene sulfide resin)
Buna-N
Operating temperature 4 °C to 50 °C
Correct Incorrect Incorrect Incorrect
Inlet Hex is Flush
with Top of Slide
Threads
Exposed
Locking Hex
Exposed
Inlet Hex
Exposed