Eppendorf XplorerPlus - Manual (Page 7)

7
Eppendorf Xplorer® (plus) — Operating manual
1.4 Glossary
A
Air-cushion
principle
An air cushion is located between the piston-cylinder system of the pipette and the liquid in the
pipette tip. Moving the piston creates a vacuum and may lead to liquid being aspirated into the
pipette tip. During the downward movement of the piston, the air cushion is pressed downwards
and the liquid in the pipette tip is dispensed. Optimizing the piston stroke ensures that the stroke
volume leads to the liquid volume shown in the display.
Pipettes in which the piston is brought into contact with the liquid to be dispensed are defined as
positive-displacement pipettes.
B
Biopur Eppendorf Biopur® is an Eppendorf AG purity level for consumables. In addition to the
requirements for standard goods, e.g., precision, accuracy, wetting behavior and leak tightness,
Biopur products also meet the highest standards when it comes to sterility and being free of ATP,
PCR inhibitors, human and bacterial DNA, pyrogens, DNase and RNase.
Goods with the Biopur purity level are inspected and certified by an external laboratory.
Lot-specific certificates can be downloaded from our website: www.eppendorf.com.
Blow-out Movement of the piston that dispenses the remaining liquid in the pipette tip after the pipetting
step has been completed. When using normal pipetting, the liquid blow-out is part of the
dispensing volume. When using reverse pipetting, blow-out is not part of the dispensing volume.
C
Cycle Together, the piston movement upward (liquid aspiration) and piston movement downward (liquid
dispensing) form a cycle.
F
Free jet dispensing Dispensing steps without a pipette tip touching tube wall. If a drip forms on the pipette tip after
free jet dispensing, this drip always belongs to the next dispensing step. We recommend
completing a dispensing series using only the free jet method or wall dispensing method.
Complete the reverse stroke in accordance with the dispensing series using either the free jet or
wall dispensing method. Errors listed in the technical data were determined using the wall
dispensing method.
I
Increment Step size or resolution. For example, the volume can be increased or decreased in 0.01 mL
increments for the volume selection on a 10 mL Xplorer pipette.
ISO 8655 The ISO 8655 standard defines, among other factors, limiting values (systematic error [accuracy]
and random error [precision]) and the test procedure for dispensing devices.
M
Multivolume The total of the remaining stroke and the reverse stroke.
N
Nominal volume The maximum dispensing volume of a pipette. The nominal volume is also used as the name of a
pipette. The term "nominal volume" comes from the ISO 8655 standard.
P
PCR clean PCR clean is an Eppendorf AG purity level for consumables. In addition to the requirements for
standard goods, e.g., precision, accuracy, wetting behavior and leak tightness, products with the
PCR clean purity level also meet the highest standards when it comes to sterility and being free
of human DNA, DNase, RNase and PCR inhibitors.
Goods with the PCR clean purity level are inspected and certified by an external laboratory.
Lot-specific certificates can be downloaded from our website: www.eppendorf.com.
PhysioCare
Concept®
Products that carry the Eppendorf PhysioCare Concept logo feature optimized ergonomics and
make operation as simple as possible, even with complex dispensing tasks. PhysioCare products
can be easily serviced and cleaned. They are made of extremely light materials and require
minimal operating force.
1
Operating instructions