Eppendorf XplorerPlus - Manual (Page 62)

Eppendorf Xplorer® (plus) — Operating manual
62
• Does the temperature of the pipetted liquid correspond to:
– The temperature of the device?
– The ambient air temperature?
• Is the weighing location free from drafts?
• Does the work method and pipetting speed permit complete aspiration and dispensing of the
liquid?
• Has the correct numerical value for "Density liquids at weighing temperature" been used for
the calculation of the actual volume?
• Is the set volume correct?
• For very small volumes (<10 μL): is the fine balance sufficiently sensitive (balance resolution:
0.001 mg)?
• Were original epT.I.P.S pipette tips used for testing?
No adjustment changes are allowed unless you can answer all the questions with "yes".
In all other cases, the problems associated with the questions answered with "no" must be
eliminated. If the problem is remedied by exchanging a complete lower part or other parts that
have an effect on the volume, proper assembly must be verified by carrying out a gravimetric test.
The systematic and random errors which must be met are listed in the technical data.
If you would like to conduct a 1-3 point adjustment for a liquid other than water on the Xplorer, the
items listed above should also be precisely observed when determining the balance results. In
particular, please note that the density of the test liquid must be precisely determined at the test
temperature. The density is dependent on the temperature. The use of an incorrect density will
lead to an incorrect volume calculation.
Hint!
The pipette tip which must be used as the test tip for checking technical data is listed in the
"Technical data" chapter in the operating manual.
The execution of the adjustment is described in the Adjustment document on the Xplorer CD.
Furthermore, use the specifications in the Standard Operating Procedure for Pipettes document
on the Xplorer CD as the foundation for determining the weighing results and errors.
CD
CD
8
General information on adjustment