9 adjustment, Adjustment, Eppendorf xplorer® (plus) — operating manual – Eppendorf XplorerPlus User Manual
Page 45: Operation
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Eppendorf Xplorer® (plus) — Operating manual
5.17.9
Adjustment
The adjustment of the Xplorer pipette can be changed to suit your needs. If you have disabled the
factory settings, the symbol and at least one additional symbol appear in the display header.
Before changing an adjustment, make sure to read the chapters "Display" (see p. 10)and
"General information about adjustment" (see p. 61).
The following adjustments can be selected:
•
Factory settings
Resets the adjustment to the factory setting.
•
Liquid type:
Ethanol 75%
The factory setting is changed by a factor in order to allow 75% ethanol to be dispensed with
higher accuracy. The internally used factor takes into account the density and the following
work technique in the Pip mode:
1. Wet the tip with liquid three times at room temperature. Hold the Xplorer pipette in an
upright position. Avoid outer wetting during dispensing.
2. Use speed level 5 (standard setting) for aspiration and dispensing.
3. Dispense liquid against the tube wall of the destination tube.
4. Trigger a blow-out (Blow) approx. 2 seconds after dispensing. Then eject the tip.
•
Liquid type:
Glycerol 50%
The factory setting is changed by a factor in order to allow 50% glycerol to be dispensed with
higher accuracy. The internally used factor takes into account the density of the 50% glycerol
and the same work technique as described previously for
Ethanol 75%
in the Pip mode.
•
epTIPS long
In addition to the "standard tips", a second longer tip is available for most Xplorer pipettes.
When
epTIPS long
is selected, the tip geometry of this longer tip is taken into account in the
internal volume calculation. This reduces the systematic error of dispensing when using these
tips.
Hint!
Changing the adjustment will change the dispensing volume and affect the accuracy of the
Xplorer pipette! Carry out a gravimetric test of the adjustment.
Every Xplorer pipette whose adjustment has been changed must be marked with a clearly
visible label that indicates the type of change made. This ensures that other users will be
informed of the changes to the adjustment, in addition to the information provided in the
header of the display.
You can overwrite a selected adjustment by selecting another adjustment. The applicable
symbols for the new adjustment will be shown in the display header (see p. 10).
For the Xplorer plus, the adjustment can be protected with a password (see Pswrd. protection
on p. 47).
Instructions on performing the adjustment can be found in the Adjustment document on the
Xplorer CD.
CD
5
Operation