Eppendorf XplorerPlus - Manual (Page 37)

37
Eppendorf Xplorer® (plus) — Operating manual
5.13.4 Reverse pipetting (Spc dial setting)
The Rev. Pipetting mode is well suited for plasma, serums and other liquids with a high protein
content. The Pipetting mode is also well suited for aqueous solutions (see p. 29). The Rev.
Pipetting mode is also well suited for solutions containing wetting agents, in order to minimize the
formation of foam during dispensing in the target tube. The liquid is aspirated using blow-out
(
Blow ). The blow-out is not part of the dispensing volume and may not be dispensed into the
target tube. If you use the same liquid again, the blow-out may remain in the tip. If you use a
different liquid, discard the blow-out and the pipette tip.
Requirement
• Pipette switched on (see p. 19)
• Rev. Pipetting (Spc) operating mode selected (see p. 33)
• Parameters set (see p. 22)
• Pipette tip attached (see p. 24)
1. Press the rocker up to aspirate the blow-out and the liquid to be dispensed.
The aspiration of the dispensing volume and blow-out will be displayed under the dispensing
volume with the label
+ Blow .
2. Press the rocker down to dispense the liquid.
The piston returns to the basic position. Liquid remains in the tip after the dispensing volume
has been dispensed.
• Press the rocker up to aspirate the same liquid.
• Press the rocker down to end the pipetting and discard the blow-out ( Blow ).
Only available for the Xplorer plus pipette
Aspiration Dispensing Aspirate or empty
Hint!
If necessary, activate the Counter setting in the (see p. 42)options to count the executed
dispensings using the pedometer.
5
Operation