Eppendorf XplorerPlus - Manual (Page 46)

Eppendorf Xplorer® (plus) — Operating manual
46
The epT.I.P.S. specified here are also available as epDualfilter T.I.P.S.
• Altitude
The aspiration volume of a piston-stroke pipette is reduced at increased altitudes, which entail
a fall in air pressure. The average air pressure at the corresponding height is used to correct
the stroke. The altitude can be selected in 250 m (820 ft) increments. 5 000 m is the
maximum altitude that can be selected.
The Xplorer pipette is set to an altitude of 0 m upon delivery. This is the factory setting.
For the following adjustments, you must know the exact density of the solution to be dispensed.
The recording of the weighing results requires the use of a fine balance with a high resolution.
Dispensing operations under 10 μL require a balance with a resolution of 0.001 mg. An existing
1-3 point adjustment can be changed.
• 1-point adjust.
After you enter the density, selected volume and corresponding weighing results, the Xplorer
pipette will determine a correction factor. The factor is only correct for the selected volume
and the selected work technique. However, the factor is used for the entire volume range of
the Xplorer pipette. You must carry out a gravimetric test to check the validity of the factor.
• 2-point adjust.
After you enter the density, two different volumes and the corresponding weighing result, the
Xplorer pipette will determine a correction factor. The factor is only correct for the selected
volume range and the selected work technique. However, the factor is used for the entire
volume range of the Xplorer pipette, i.e., below and above the two measuring points as well.
You must carry out a gravimetric test to check the validity of the factor.
Xplorer
Nominal volume
Volume range Color code
Xplorer rocker The adjustment for epTIPS long applies only to
Color code
epT.I.P.S. Model
epT.I.P.S. Length
epT.I.P.S.
10 μL
0.5 - 10 μL medium gray light gray 20 μL Long 46 mm
100 μL
5 - 100 μL yellow orange 300 μl 55 mm
300 μL
15 - 300 μL orange not available
1 000 μL
50 - 1 000 μL blue dark green 1 250 μL Long 103 mm
1 200 μL
50 - 1 200 μL green dark green 1 250 μL Long 103 mm
5 mL
0.25 – 5 mL purple not available
10 mL
0.5 – 10 mL turquoise turquoise 10 mL Long 243 mm
Hint!
You can select volumes 0.2 mL for the 5 mL Xplorer. No technical data has been specified for
the 0.2 mL – 0.5 mL volume range.
Hint!
The liquid type ( Ethanol 75% or Glycerol 50% ), epTIPS long and Altitude options can be
combined.
5
Operation