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1 autoclaving, 2 disinfection, 3 replacing o-rings – Eppendorf XplorerPlus User Manual

Page 52: Autoclaving, Disinfection, Replacing o-rings, Eppendorf xplorer® (plus) — operating manual

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Eppendorf Xplorer® (plus) — Operating manual

52

7.2.1

Autoclaving

All Xplorer lower parts are steam autoclavable.

Before autoclaving

1. Remove any contamination from the exterior and in the lower part (see Xplorer pipette

disassembly and assembly on p. 53).

2. If you remove grease, only relubricate the piston seal slightly with the grease specified in the

ordering information (see the Xplorer CD).

Procedure

Follow the operating manual of the autoclave manufacturer.

1. Autoclave at 121°C, 20 minutes, 1 bar pressure.
2. Put the lower part into the autoclave in such a way to ensure that the temperature at the lower

part does not exceed 121°C.

3. You can put the lower part into the autoclave as a whole unit or as individual parts.
4. Make sure that no parts of disassembled lower parts are confused during subsequent

assembly (recommendation: use a plastic beaker for each lower part).

After autoclaving

1. Cool the lower part down to room temperature and leave to dry. The piston does not need to

be greased following autoclaving.

2. Carry out a gravimetric test to check that the Xplorer pipette is functioning.

7.2.2

Disinfection

1. Carefully wipe off the outer surfaces with disinfectant, DNA/RNA decontamination agents or

70% isopropanol.

2. You can wipe the outside of the tip cone and the ejector sleeve with a sodium hypochlorite

solution of 4%.

3. After the exposure time of the sodium hypochlorite solution has elapsed, thoroughly remove it

using demineralized water.

7.3

Replacing O-rings

The 100 μL, 300 μL and 1 200 μL multi-channel lower parts are equipped with O-rings. These
are wear parts. Replace old, worn or damaged O-rings. Defective O-rings result in the incorrect
positioning of the tips and in dispensing errors.

7

Maintenance

This manual is related to the following products: