Eppendorf XplorerPlus - Manual (Page 29)

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Eppendorf Xplorer® (plus) — Operating manual
5.10 Pipetting (Pip dial setting)
5.10.1 Standard pipetting
Aspirate a liquid and then dispense it again in the Pipetting (Pip) mode. The blow-out can be
performed manually or automatically using the dispensing step.
Requirement
• Pipette switched on (see p. 19)
• Pipetting (Pip) operating mode selected (see p. 33)
• Parameters set (see p. 22)
• Pipette tip attached (see p. 24)
1. Press the rocker up to aspirate liquid.
2. Press the rocker down to dispense the liquid.
If dispensing and blow-out (Blow) are carried out in a single step, hold the rocker pressed
down during dispensing.
3. If you keep the rocker pressed during the blow-out (Blow), the piston moves to the lowest
position and remains there until the rocker is released.
Aspiration Dispensing Blow-out
Hint!
Hold the rocker pressed down to trigger dispensing and blow-out (Blow) in a single step.
Hint!
Dispense the liquid against the tube inner wall if you are determining the systematic and random
errors.
Hint!
If you only hold the rocker pressed down during the blow-out (Blow) after dispensing, the piston
remains in the lowest position. The piston will only return to the basic position after the rocker has
been released. This presents the following advantages:
 If you pipette liquid that drains slowly, you can allow this liquid to flow out.
 You can immerse the pipette tip in the target liquid to prevent unintentional aspiration.
Hint!
 You can trigger the blow-out (Blow) any time the piston is in the basic position. Press the
rocker down.
 Press the rocker in the opposite direction if you would like to stop aspiration or dispensing
while it is in progress. The display shows the volume present in the pipette tip after the
respective operation has been stopped. You can then continue with liquid aspiration or
dispensing.
 The number of dispensing steps performed can be counted with a counter ( Counter ). The
counter can be activated ( Counter ) if desired (see p. 42).
5
Operation