Eppendorf XplorerPlus - Manual (Page 30)

Eppendorf Xplorer® (plus) — Operating manual
30
5.10.2 Reverse pipetting
Reverse pipetting is advantageous for plasma, serum and other liquids with a high protein
content. Reverse pipetting is not necessary for pipetting aqueous solutions. By using the
blow-out, more liquid is aspirated with reverse pipetting than with standard pipetting. These
additional volumes may result in volume limits when using filter tips (see p. 43). During
dispensing, the blow-out (Blow) does not belong to the dispensing volume.
Requirement
• Pipette switched on (see p. 19)
• Pipetting (Pip) operating mode selected (see p. 33)
• Parameters set (see p. 22)
• Pipette tip attached (see p. 24)
1. Keep the rocker pressed down.
2. Immerse the pipette tip in the liquid.
3. Release the rocker to aspirate the blow-out.
4. Press the rocker up to aspirate the dispensing volume.
5. Place the pipette tip on the wall of the target tube.
6. Press the rocker down to dispense the liquid.
7. Remove the pipette tip from the destination tube. Residual liquid remains in the tip.
There are two courses of further action:
Aspirate the same liquid from the same reservoir tube
 Press the rocker up to aspirate the same liquid. The residual liquid in the tip is used for further
processing.
Dispense a different liquid
1. Press the rocker up to discard the residual liquid in the tip.
2. Press the ejector to eject the pipette tip.
Use a new pipette tip for the next dispensing operation.
Use the Rev. Pipetting operating mode for the Xplorer plus pipette (see p. 37).
Keep the
rocker
pressed
down
Release the
rocker
Aspirate
blow-out
Aspiration Dispensing Aspirate or
empty
5
Operation