2 volume limit, Volume limit, See p. 43) – Eppendorf XplorerPlus User Manual
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43
Eppendorf Xplorer® (plus) — Operating manual
5.17.2
Volume limit
This limits the aspiration volume in all dispensing modes.
Use the volume limit in the following cases:
•
For strongly foaming solutions in order to prevent contamination of the tip cone or the
protection filter.
•
When using ep Dualfilter T.I.P.S. in the Ads, Dis and Pip modes (reverse pipetting) and the
Xplorer plus
Rev. Pipetting
mode. For these three versions, there is a risk of the liquid coming
into contact with the dualfilter because of the additional volumes during aspiration.
•
For pipette tips or filter tips whose aspiration volume is smaller than the nominal volume of the
Xplorer pipette.
Other volume limits may apply when using filter tips from other manufacturers. The volume limit
differs depending on the tip geometry and the position of the filter in the tip.
The volume limit is dependent on the additional aspirated volumes of the Xplorer pipette. On the
Xplorer the individual nominal volumes result in different additional volumes.
Hint!
After a volume limit has been set, a note will be shown in the display when the Pip, P/M and
Man operating modes are edited and when the volume limit has been reached. In this mode,
too large dispensing volumes will be automatically reduced to the defined volume limit. The
number of potential dispensing steps will be automatically limited in the Dis and Ads modes.
If the corresponding volume is above the defined volume limit, saved dispensing can no
longer be executed under
History
and under the Spc, Prg and Fix modes. The volume can be
changed in the corresponding mode or the Edit mode.
ep Dualfilter T.I.P.S.
Volume limit
Ads/Dis
Reverse pipetting
10 μL
Not required
Not required
100 μL
Not required
Not required
200 μL
Not required
Not required
300 μl
270 μL
260 μL
1 000 μL
Not required
950 μL
1 250 μL Long*
Not required
1 130 μL
5 mL
4.9 mL
4.6 mL**
10 mL*
9.75 mL
8.5 mL**
* When using these filter tips we recommend using the
epTIPS
long
adjustment to increase the accuracy of dispensing. The
specified volume limit only applies to the changed adjustment.
** We also recommend this volume limit for "Reverse Pipetting"
when using 5 mL and 10 mL epT.I.P.S. without filter.
5
Operation