Eppendorf XplorerPlus - Manual (Page 34)

Eppendorf Xplorer® (plus) — Operating manual
34
5.13.2 Diluting (Spc dial setting)
Two different liquids are aspirated in the Diluting mode. These liquids are separated by an air
bubble. The Diluting mode is suitable for diluting samples and reagents using an appropriate
dilution solution (diluent). First, the diluent is aspirated, then the air bubble, and finally the sample
or reagent.
Requirement
• Pipette switched on (see p. 19)
• Diluting (Spc) operating mode selected (see p. 33)
• Parameters set (see p. 22)
• Pipette tip attached (see p. 24)
1. Press the rocker up to aspirate the diluent.
2. Press the rocker up to aspirate the air bubble.
3. Press the rocker up to aspirate the sample or reagent.
4. Press the rocker down to dispense all of the liquid.
5. Press the rocker down to trigger the blow-out
Blow .
6. If the piston is in the basic position: press the Back softkey to exit the mode.
Examples:
Required auxiliary aids:
• Xplorer plus with a nominal volume of 1 000 μL
• Dispensing tube (1.5 mL)
Only available for the Xplorer plus pipette
Hint!
Because both liquids are aspirated in a single pipette tip, the sample or reagent may be slightly
contaminated by the dilution solution.
Diluent Aspirating the air
bubble
Aspiration of the
sample or reagent
Dispensing
Hint!
For execution, the parameter sequence is carried out in the reverse order of the editing process.
The next volume to be aspirated will be shown in the display with a border around it.
Hint!
Set a high value for the Dispensing speed parameter to achieve a good mixture for the
dispensing.
Hint!
To create a very high mixture, you can enter the "Diluting" mode with the Pipetting and Mixing
mode (P/M) as the program (see p. 39).
5
Operation