Eppendorf XplorerPlus - Manual (Page 25)

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Eppendorf Xplorer® (plus) — Operating manual
5.7 Tips for correct pipetting
To achieve the highest precision and accuracy, we recommend observing the following hints:
5.7.1 Preparation
 Always place the liquid in a suitable tube. For multi-channel pipettes, we recommend reagent
reservoir Tip-Tub as a liquid reservoir. The liquid and the pipette should have approximately
the same temperature.
 Use epT.I.P.S. To minimize residual moisture in the tip after dispensing, LoRetention for
solutions containing surfactants.
 Use ep Dualfilter T.I.P.S. to prevent aerosol contamination in the tip.
 Use the pipette tips only once.
 Check whether the set aspiration and dispensing speeds are suitable for the liquid. The
blow-out (Blow) will be conducted at approximately the same speeds.
5.7.2 Aspirating liquid
5.7.3 Dispensing liquid
Reagent reservoir Tip-Tub
1. Wet the new pipette tip by aspirating and dispensing the liquid to be pipetted one to three
times.
2. Vertically immerse the pipette tip into the liquid approx. 4 mm during aspiration.
3. Press the rocker up to aspirate liquid. Maintain the immersion depth, to ensure no air is
accidentally aspirated.
4. For large volumes, keep the pipette tips in the liquid for approx. 3 seconds after aspiration.
5. After aspiration, slowly pull the pipette tip out of the liquid.
6. Wipe the pipette tip slowly against the tube wall to ensure that no outer wetting remains on the
pipette tip.
1. When dispensing liquid, place the pipette tip at a slight angle against the wall of the tube.
2. Press the rocker down to dispense the liquid.
3. After dispensing, wait until no more liquid is dispensed.
4. Press the rocker down again to trigger the blow-out (Blow).
5
Operation