Eppendorf XplorerPlus - Manual (Page 28)

Eppendorf Xplorer® (plus) — Operating manual
28
5.9 Dispensing (Dis dial setting)
In the Dispensing (Dis) mode, liquid is aspirated and individually dispensed in uniform partial
volumes. The reverse stroke and remaining stroke are triggered before or after the dispensing
steps. These additional volumes may result in volume limits when using filter tips (see p. 43).
Requirement
• Pipette switched on (see p. 19)
• Dispensing (Dis) operating mode selected (see p. 33)
• Parameters set (see p. 22)
• Pipette tip attached (see p. 24)
1. Press the rocker up to aspirate liquid.
2. Press the rocker down to trigger the reverse stroke.
3. Press the rocker down again for each dispensing operation. The display indicates the
remaining dispensing steps under Steps .
After dispensing, decide whether the residual liquid should be dispensed or liquid should be
aspirated again.
 Liquid aspiration: press the rocker up once.
 Liquid dispensing: press the rocker down twice.
Aspiration Reverse stroke Dispensing Aspirate or
empty
1 2 3 4
Hint!
 Press the Empty softkey to cancel dispensing. The tip is completely emptied.
 Press the rocker up to stop a current dispensing of a large dispensing volume.
Hint!
• Free jet dispensing may be used to dispense aqueous solutions with a volume of
approximately 20 μL or more. We recommend wall dispensing for dispensing aqueous
solutions with a smaller volume.
• Select level 8 for the Speed parameter when using use free jet dispensing and if the geometry
of the dispensing tube permits this action.
• If you dispense liquid in the free jet, the reverse stroke must also be dispensed in the free jet.
If a drop then forms on the pipette tip, it is included in the first dispensing volume.
• For wall dispensing, trigger the reverse stroke with the pipette tips on the tube inner wall.
• During dispensing, do not switch between free jet dispensing and wall dispensing.
5
Operation