Eppendorf XplorerPlus - Manual (Page 36)

Eppendorf Xplorer® (plus) — Operating manual
36
5.13.3 Sequential dispensing (Spc dial setting)
Up to 10 different volumes can be dispensed in the Seq. Dispensing mode. The sum of the
dispensing volumes may not exceed the pipette tip filling volume. This mode is well suited for
dilution series. The
Seq. Pipetting mode is also well suited for dilution series. To conduct a
dilution series, dispense two different liquids in the opposite direction. This allows you to achieve
different dilutions with the same volume per tube.
Requirement
• Pipette switched on (see p. 19)
• Seq. Dispensing (Spc) operating mode selected (see p. 33)
• Parameters set (see p. 22)
• Pipette tip attached (see p. 24)
1. Press the rocker up to aspirate liquid.
2. Press the rocker down to execute the reverse stroke.
3. Press the rocker down for each dispensing step.
4. If the piston is in the basic position: press the
Back softkey to exit the mode.
Example
Required auxiliary aids:
• Xplorer plus with a nominal volume of 300 μL
• 4 dispensing tubes (200 μL)
Only available for the Xplorer plus pipette
Hint!
If the sum of the dispensing volumes exceeds the volume of the pipette tip: create a program and
execute the mode several times in a row.
Tube 1 Tube 2 Tube 3 Tube 4
Volume/step 30 μL 60 μL 90 μL 120 μL
Volume/step
120 μL 90 μL 60 μL 30 μL
Dilution 1+X 1+4 1+1.5 1+0.67 1+0.25
Dilution 1:Y 1:5 1:2.5 1:1.67 1:1.25
Tube sum 150 μL 150 μL 150 μL 150 μL
1 2 3 4 6 5
1
1 2 3 4 6 5
2
5
Operation