Required laboratory reagents, Software, Luminex 200 performance specifications – Luminex 200 User Manual with LDS 1.7 Software User Manual
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Luminex 200 User Manual for LDS Version 1.7
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MAP Technology
3 - 2
PN 89-00002-00-150 Rev. A
Required
Laboratory
Reagents
The following items are necessary to run the Luminex 200 system.
They are not provided by Luminex.
•
Household bleach
•
70% isopropanol or 70% ethanol
•
Mild detergent
•
Distilled water
Software
Luminex Data Collector software provides complete control of the
Luminex analyzer and performs real-time digital analysis of assays.
The Luminex 200 system is preloaded with the Luminex software.
However, we supply a software CD in case you need to reinstall the
software.
This software requires a dedicated system. Unauthorized additional
software is prohibited and may result in improper operation of the
system.
Luminex 200
Performance
Specifications
Speed
•
USB communications link for fast data transfer
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Automatic transfer of assay templates and new reagent
information into the system via a large capacity read/write CD
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Installation: < 4 hours
•
System calibration: < 10 minutes
•
Analyze one 96-well plate/hour depending on manufacturer’s kit
•
Up to 100 xMAP microsphere sets per sample
•
System warmup: 30 minutes. Systems that remain inactive for at
least four hours will require a warm-up to restart the lasers. After
acquiring sample, running system calibrators, running system
controls, and warming up the instrument, the system resets the
four-hour internal clock.
Accuracy and Precision
•
Sample uptake volume: ± 5%
•
Classification of xMAP microspheres: > 80%
•
Misclassification of xMAP microspheres:
≤ 2% - may vary by
xMAP microsphere product lines. Refer to the specific product
information sheet for further details.
•
Internal sample carry over: < 0.9%
•
Soluble background fluorescence emission at 575 nm
automatically subtracted from fluorescence intensity values